RIPK3 Antibody

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ProSci
Product Code: PSI-57-813
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-813-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:500 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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Western Blot at 1:500 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Receptor-interacting serine/threonine-protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, RIPK3, RIP3
Application Note:
For WB starting dilution is: 1:500

For IHC-P starting dilution is: 1:10~50
Background:
The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Background References:
  • Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
  • Vandenabeele, P., et al. Sci Signal(115), RE4 (2010) :
  • Cho, Y.S., et al. Cell 137(6):1112-1123(2009)
  • He, S., et al. Cell 137(6):1100-1111(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-509 amino acids from the C-terminal region of human RIPK3.
NCBI Gene ID #:
11035
NCBI Official Name:
Receptor-interacting serine/threonine-protein kinase 3
NCBI Official Symbol:
RIPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q9Y572
Protein GI Number:
205371831
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Immunology,Signal Transduction
Swissprot #:
Q9Y572
User NOte:
Optimal dilutions for each application to be determined by the researcher.