CER1 Antibody

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ProSci
Product Code: PSI-62-558
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-558-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CER1 Antibody in NCI-H460 cell line lysates (35ug/lane)
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(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CER1 Antibody in NCI-H460 cell line lysates (35ug/lane)
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Cerberus, Cerberus-related protein, DAN domain family member 4, CER1, DAND4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.
Background References:
  • Tang,P.L., et.al., J. Bone Miner. Res. 24 (6), 1013-1021 (2009)
  • Swinkels,M.E., et.al., Am. J. Med. Genet.146A (11), 1430-1438 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human CER1.
NCBI Gene ID #:
9350
NCBI Official Name:
Cerberus
NCBI Official Symbol:
CER1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
O95813
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Immunology,Signal Transduction
Swissprot #:
O95813
User NOte:
Optimal dilutions for each application to be determined by the researcher.