SERPINA3 Antibody

ProSci
Product Code: PSI-62-816
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-816-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
2 / 2
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3, Alpha-1-antichymotrypsin His-Pro-less, SERPINA3, AACT
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SERPINA3 is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Background References:
  • Abraham,C.R., Shirahama,T. Neurobiol. Aging 11 (2), 123-129 (1990)
  • Baumann,U., Huber,R. J. Mol. Biol. 218 (3), 595-606 (1991)
  • Desrochers,P.E., Mookhtiar,K. J. Biol. Chem. 267 (7), 5005-5012 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SERPINA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-310 amino acids from the C-terminal region of human SERPINA3.
NCBI Gene ID #:
12
NCBI Official Name:
Alpha-1-antichymotrypsin
NCBI Official Symbol:
SERPINA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P01011
Protein GI Number:
112874
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
P01011
User NOte:
Optimal dilutions for each application to be determined by the researcher.