NDN Antibody

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ProSci
Product Code: PSI-58-061
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-061-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Ramos cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in Ramos cell line lysates (35ug/lane).
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Necdin, NDN
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.
Background References:
  • Chapman, E.J., et al. Mol. Carcinog. 48(11):975-981(2009)
  • Yoshikawa, K. No To Hattatsu 41(3):214-218(2009)
  • Liu, X., et al. Mol. Cell. Neurosci. 41(1):51-61(2009)
  • de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NDN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human NDN.
NCBI Gene ID #:
4692
NCBI Official Name:
Necdin
NCBI Official Symbol:
NDN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q99608
Protein GI Number:
17380142
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
Q99608
User NOte:
Optimal dilutions for each application to be determined by the researcher.