YWHAB Antibody

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ProSci
Product Code: PSI-63-435
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-435-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Antibody is used in Western blot to detect YWHAB in Jurkat cell lysate.
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Confocal immunofluorescent analysis of YWHAB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect YWHAB in Jurkat cell lysate.
Confocal immunofluorescent analysis of YWHAB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha, N-terminally processed, YWHAB
Application Note:
For IHC-P starting dilution is: 1:50~100

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Background References:
  • Komori, T., et al., Acta Neuropathol. 106(1):66-70 (2003). Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (2003). Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003). Shumway, S.D., et al., J. Biol. Chem. 278(4):2089-2092 (2003). Parvaresch, S., et al., FEBS Lett. 532(3):357-362 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Rat, Zebrafish
Immunogen:
This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human YWHAB.
NCBI Gene ID #:
7529
NCBI Official Name:
14-3-3 protein beta/alpha
NCBI Official Symbol:
YWHAB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
P31946
Protein GI Number:
1345590
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P31946
User NOte:
Optimal dilutions for each application to be determined by the researcher.