SOX9 Antibody

ProSci
Product Code: PSI-57-473
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-473-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-SOX9 Antibody in K562 cell line lysates (35ug/lane)
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Western blot analysis of SOX9 using rabbit polyclonal SOX9 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2).
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Immunofluorescence analysis of anti-SOX9 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Western blot analysis of anti-SOX9 Antibody in K562 cell line lysates (35ug/lane)
Western blot analysis of SOX9 using rabbit polyclonal SOX9 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2).
Immunofluorescence analysis of anti-SOX9 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Further Information

Additional Names:
Transcription factor SOX-9, SOX9
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Background References:
  • Malki,S., Cancer Lett. 255 (2), 182-193 (2007)
  • Passeron,T., Proc. Natl. Acad. Sci. U.S.A. 104 (35), 13984-13989 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig
Immunogen:
This SOX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human SOX9.
NCBI Gene ID #:
6662
NCBI Official Name:
Transcription factor SOX-9
NCBI Official Symbol:
SOX9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
P48436
Protein GI Number:
1351096
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P48436
User NOte:
Optimal dilutions for each application to be determined by the researcher.