NFE2L2 Antibody

ProSci
Product Code: PSI-62-788
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-788-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-NFE2L2 Antibody in Ramos cell line lysates (35ug/lane)
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Western blot analysis of Nrf2(Nfe2l2) using rabbit polyclonal Nrf2(Nfe2l2) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2).

Western blot analysis of anti-NFE2L2 Antibody in Ramos cell line lysates (35ug/lane)
Western blot analysis of Nrf2(Nfe2l2) using rabbit polyclonal Nrf2(Nfe2l2) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2).

Further Information

Additional Names:
Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2, NRF2
Application Note:
For WB starting dilution is: 1:1000
Background:
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995).
Background References:
  • Zhao,X., Stroke 38 (12), 3280-3286 (2007) Li,M.H., J. Biol. Chem. 282 (39), 28577-28586 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NFE2L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human NFE2L2.
NCBI Gene ID #:
4780
NCBI Official Name:
Nuclear factor erythroid 2-related factor 2
NCBI Official Symbol:
NFE2L2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q16236
Protein GI Number:
25453452
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q16236
User NOte:
Optimal dilutions for each application to be determined by the researcher.