FOXC2 Antibody

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ProSci
Product Code: PSI-55-926
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-926-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in WiDr,HL-60,ZR-75-1,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXC2 antibody detected the FOXC2 protein (arrow).
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in WiDr,HL-60,ZR-75-1,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXC2 antibody detected the FOXC2 protein (arrow).

Documents

Further Information

Additional Names:
Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH-1 protein, Transcription factor FKH-14, FOXC2, FKHL14, MFH1
Application Note:
For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.
Background References:
  • Sanchez-Carpintero, R., et al. Genet. Med. 12(8):532-535(2010)
  • Pappa, K.I., et al. Gynecol. Endocrinol. (2010) In press :
  • Mortazavi, F., et al. Mol. Cancer Res. 8(5):762-774(2010)
  • Fauret, A.L., et al. Lymphology 43(1):14-18(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FOXC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human FOXC2.
NCBI Gene ID #:
2303
NCBI Official Name:
Forkhead box protein C2
NCBI Official Symbol:
FOXC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q99958
Protein GI Number:
3024149
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q99958
User NOte:
Optimal dilutions for each application to be determined by the researcher.