FLI1 Antibody

ProSci
Product Code: PSI-62-291
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-291-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of FLI1 antibody in 293 cell line lysates (35ug/lane)
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FLI-1 Antibody IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of FLI1 antibody in 293 cell line lysates (35ug/lane)
FLI-1 Antibody IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
FLI1 is a sequence-specific transcriptional activator. It recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Background References:
  • Embree,L.J., Cancer Res. 69 (10), 4363-4371 (2009)
  • Torlakovic,E.E., Histol. Histopathol. 23 (11), 1309-1314 (2008)
  • Cironi,L., PLoS ONE(7), E2634 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This FLI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-80 amino acids from the N-terminal region of human FLI1.
NCBI Gene ID #:
2313
NCBI Official Name:
Friend leukemia integration 1 transcription factor
NCBI Official Symbol:
FLI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q01543
Protein GI Number:
399496
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q01543
User NOte:
Optimal dilutions for each application to be determined by the researcher.