MAFF Antibody

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ProSci
Product Code: PSI-62-492
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-492-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human skin reacted with MAFF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:1000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human skin reacted with MAFF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. This protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription.
Background References:
  • Kataoka,K., et.al., J. Biol. Chem. 276 (1), 819-826 (2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MAFF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-92 amino acids from the Central region of human MAFF.
NCBI Gene ID #:
23764
NCBI Official Name:
Transcription factor MafF
NCBI Official Symbol:
MAFF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
Q9ULX9
Protein GI Number:
21542145
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q9ULX9
User NOte:
Optimal dilutions for each application to be determined by the researcher.