ELMO2 Antibody

ProSci
Product Code: PSI-55-631
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-631-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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ELMO2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
ELMO2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Engulfment and cell motility protein 2, Protein ced-12 homolog A, hCed-12A, ELMO2, CED12A, KIAA1834
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein.
Background References:
  • Bento, J.L., et al. Genomics 92(4):226-234(2008)
  • Rikova, K., et al. Cell 131(6):1190-1203(2007)
  • Zhang, Y., et al. Mol. Cell Proteomics 4(9):1240-1250(2005)
  • Zhang, Y., et al. Mol. Cell Proteomics 4(9):1240-1250(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ELMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-350 amino acids from the Central region of human ELMO2.
NCBI Gene ID #:
63916
NCBI Official Name:
Engulfment and cell motility protein 2
NCBI Official Symbol:
ELMO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
Q96JJ3
Protein GI Number:
30913107
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q96JJ3
User NOte:
Optimal dilutions for each application to be determined by the researcher.