PHB Antibody

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ProSci
Product Code: PSI-61-438
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-438-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Prohibitin, PHB
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
Background References:
  • Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008)
  • Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008)
  • White,J.J., Genomics 11 (1), 228-230 (1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken
Immunogen:
This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
NCBI Gene ID #:
5245
NCBI Official Name:
Prohibitin
NCBI Official Symbol:
PHB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P35232
Protein GI Number:
464371
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity
Swissprot #:
P35232
User NOte:
Optimal dilutions for each application to be determined by the researcher.