HFE2 Antibody

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ProSci
Product Code: PSI-64-070
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-070-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from human skeletal muscle and heart tissue lysates (from left to right), using HFE2 Antibody at 1:1000 at each lane.
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HFE2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysates from human skeletal muscle and heart tissue lysates (from left to right), using HFE2 Antibody at 1:1000 at each lane.
HFE2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Hemojuvelin, Hemochromatosis type 2 protein, RGM domain family member C, HFE2, HJV, RGMC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
HFE2 is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin.
Background References:
  • Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
  • Barton, J.C., et al. Am. J. Hematol. 84(11):710-714(2009)
  • Zhang, A.S., et al. J. Biol. Chem. 284(34):22580-22589(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-162 amino acids from the Central region of human HFE2.
NCBI Gene ID #:
148738
NCBI Official Name:
Hemojuvelin
NCBI Official Symbol:
HFE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
Q6ZVN8
Protein GI Number:
51316254
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Neuroscience,Signal Transduction
Swissprot #:
Q6ZVN8
User NOte:
Optimal dilutions for each application to be determined by the researcher.