SMPD1 Antibody

Product Code: PSI-56-289

Supplier: ProSci

Code	Size	Price

PSI-56-289-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Fluorescence-activated cell sorting (FACS)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti--Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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Western Blot at 1:2000 dilution Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

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Documents

Further Information

Additional Names:

Sphingomyelin phosphodiesterase, Acid sphingomyelinase, aSMase, SMPD1, ASM

Application Note:

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50

Background:

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq].

Background References:

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Desnick, J.P., et al. Mol. Med. 16 (7-8), 316-321 (2010)Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) Sleat, D.E., et al. Mol. Cell Proteomics 5(4):686-701(2006)

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Bovine

Immunogen:

This SMPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids from the C-terminal region of human SMPD1.

NCBI Gene ID #:

6609

NCBI Official Name:

Sphingomyelin phosphodiesterase

NCBI Official Symbol:

SMPD1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

70 kDa

Protein Accession #:

P17405

Protein GI Number:

224471897

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Research Area:

Cell Cycle,Obesity,Neuroscience,Signal Transduction

Swissprot #:

P17405

User NOte:

Optimal dilutions for each application to be determined by the researcher.