ADORA2A Antibody

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ProSci
Product Code: PSI-55-195
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-195-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of ADORA2A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of ADORA2A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Adenosine receptor A2a, ADORA2A, ADORA2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
Background References:
  • Buira, S.P., et al. J. Neurochem. 115(1):283-295(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Rogers, P.J., et al. Neuropsychopharmacology 35(9):1973-1983(2010)
  • Tebano, M.T., et al. ScientificWorldJournal 10, 1768-1782 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ADORA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the Central region of human ADORA2A.
NCBI Gene ID #:
135
NCBI Official Name:
Adenosine receptor A2a
NCBI Official Symbol:
ADORA2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P29274
Protein GI Number:
543740
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Neuroscience,Signal Transduction,Growth Factor
Swissprot #:
P29274
User NOte:
Optimal dilutions for each application to be determined by the researcher.