TREF1 Antibody

Contact us
ProSci
Product Code: PSI-63-909
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-909-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Fluorescent image of MCF-7 cell stained with TREF1 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TREF1 immunoreactivity is localized to Nucleus significantly.
2 / 3
Western blot analysis of TREF1 Antibody in K562 cell line lysates (35ug/lane)
3 / 3
❮❯

Fluorescent image of MCF-7 cell stained with TREF1 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TREF1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis of TREF1 Antibody in K562 cell line lysates (35ug/lane)

Documents

Further Information

Additional Names:
Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132, TRERF1, BCAR2, RAPA, TREP132
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
TREF1 encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1.
Background References:
  • Gizard,F. Mol. Cell. Biol. 26 (20), 7632-7644 (2006)
  • Gizard,F. Mol. Cell. Biol. 25 (11), 4335-4348 (2005)
  • Beausoleil,S.A. Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004)
  • Gizard,F. J. Mol. Endocrinol. 32 (2), 557-569 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TREF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1136-1164 amino acids from the C-terminal region of human TREF1.
NCBI Gene ID #:
55809
NCBI Official Name:
Transcriptional-regulating factor 1
NCBI Official Symbol:
TRERF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
132 kDa
Protein Accession #:
Q96PN7
Protein GI Number:
74762683
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q96PN7
User NOte:
Optimal dilutions for each application to be determined by the researcher.