MLK2 Antibody

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ProSci
Product Code: PSI-63-288
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-288-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Antibody is used in Western blot to detect MLK2 in transfected COS7 cell lysate. Data is kindly provided by Drs. M.K. Barthwal and A. Rana from Texas A&M University, Health Science Center (Temple, TX).
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Antibody is used in Western blot to detect MLK2 in transfected COS7 cell lysate. Data is kindly provided by Drs. M.K. Barthwal and A. Rana from Texas A&M University, Health Science Center (Temple, TX).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase 10, Mixed lineage kinase 2, Protein kinase MST, MAP3K10, MLK2, MST
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
MLK2 is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.
Background References:
  • Dorow, D.S., et al., Eur. J. Biochem. 234(2):492-500 (1995).
  • Katoh, M., et al., Oncogene 10(7):1447-1451 (1995).
  • Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-659 amino acids from the C-terminal region of human MLK2.
NCBI Gene ID #:
4294
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase 10
NCBI Official Symbol:
MAP3K10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
104 kDa
Protein Accession #:
Q02779
Protein GI Number:
145559494
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q02779
User NOte:
Optimal dilutions for each application to be determined by the researcher.