HSF1 Antibody

ProSci
Product Code: PSI-57-547
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-547-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HSF1 using rabbit polyclonal HSF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.
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HSF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of HSF1 using rabbit polyclonal HSF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.
HSF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.
Background References:
  • Hayashida, N., et al. EMBO J. 29(20):3459-3469(2010)
  • Sangle, G.V., et al. Endocrinology 151(9):4455-4466(2010)
  • Shunmei, E., et al. Mol. Cells 29(5):527-531(2010)
  • Kim, S.A., et al. Int. J. Oncol. 36(4):867-872(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Xenopus
Immunogen:
This HSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the N-terminal region of human HSF1.
NCBI Gene ID #:
3297
NCBI Official Name:
Heat shock factor protein 1
NCBI Official Symbol:
HSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q00613
Protein GI Number:
462333
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q00613
User NOte:
Optimal dilutions for each application to be determined by the researcher.