TXNL1 Antibody

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ProSci
Product Code: PSI-57-017
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-017-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect TrxL in HL-60 cell lysate.
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Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.
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Antibody is used in Western blot to detect TrxL in HL-60 cell lysate.
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.

Documents

Further Information

Additional Names:
Thioredoxin-like protein 1, 32 kDa thioredoxin-related protein, TXNL1, TRP32, TXL, TXNL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Thioredoxins are a group of small redox active proteins possessing a conserved active site sequence. TrxL (Thioredoxin like protein 1) has 2 distinct domains: an N terminal domain, which is 43% identical to human TXN, and a C terminal domain, which shows no homology to other proteins in the sequence databases. The active site sequence, located within the N terminal domain, is that of a thioredoxin like protein; compared to the active site sequence of TXN, it has a single amino acid substitution. Unlike other thioredoxin like proteins, TrxL does not serve as a substrate for thioredoxin reductase in an insulin assay.
Background References:
  • Miranda-Vizuete, A., et al., Biochem. Biophys. Res. Commun. 243: 284-288 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This TXNL1 (TRP32/TrxL) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-289 amino acids from the C-terminal region of human TXNL1 (TRP32/TrxL).
NCBI Gene ID #:
9352
NCBI Official Name:
Thioredoxin-like protein 1
NCBI Official Symbol:
TXNL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
O43396
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
O43396
User NOte:
Optimal dilutions for each application to be determined by the researcher.