PDXK Antibody
| Code | Size | Price |
|---|
| PSI-62-699-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
Background References:
- Shin, J.H., et al., Neurochem. Int. 45(1):73-79 (2004).
- Lee, H.S., et al., Mol. Cells 10(4):452-459 (2000).
- Laine-Cessac, P., et al., Biochem. Pharmacol. 54(8):863-870 (1997).
- Hanna, M.C., et al., J. Biol. Chem. 272(16):10756-10760 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDXK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-272 amino acids from the C-terminal region of human PDXK.
NCBI Gene ID #:
8566
NCBI Official Name:
Pyridoxal kinase
NCBI Official Symbol:
PDXK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
O00764
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
O00764
User NOte:
Optimal dilutions for each application to be determined by the researcher.