AKR1A1 Antibody

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ProSci
Product Code: PSI-61-464
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-464-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-AKR1A1 Antibody in Y79 cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-AKR1A1 Antibody in Y79 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Alcohol dehydrogenase [NADP(+)], Aldehyde reductase, Aldo-keto reductase family 1 member A1, AKR1A1, ALDR1, ALR
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
AKR1A1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.
Background References:
  • Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008)
  • Bohren,K.M.,Biochim. Biophys. Acta 1748 (2), 201-212 (2005)
  • El-Kabbani,O.,Acta Crystallogr.Biol. Crystallogr. 50 (PT 6), 859-868 (1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This AKR1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-325 amino acids from the C-terminal region of human AKR1A1.
NCBI Gene ID #:
10327
NCBI Official Name:
Alcohol dehydrogenase [NADP(+)]
NCBI Official Symbol:
AKR1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
P14550
Protein GI Number:
113600
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P14550
User NOte:
Optimal dilutions for each application to be determined by the researcher.