GLYAT Antibody

ProSci
Product Code: PSI-56-828
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-828-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in 293 cell line lysates (35ug/lane).
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GLYAT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in 293 cell line lysates (35ug/lane).
GLYAT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, AAc, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferase, HRP-1(CLP), GLYAT, ACGNAT, CAT, GAT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Yamamoto, A., et al. Drug Metab. Pharmacokinet. 24(1):114-117(2009)
  • Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
  • van der Westhuizen, F.H., et al. J. Biochem. Mol. Toxicol. 14(2):102-109(2000)
  • Mawal, Y., et al. J. Pediatr. 130(6):1003-1007(1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GLYAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human GLYAT.
NCBI Gene ID #:
10249
NCBI Official Name:
Glycine N-acyltransferase
NCBI Official Symbol:
GLYAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q6IB77
Protein GI Number:
311033446
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q6IB77
User NOte:
Optimal dilutions for each application to be determined by the researcher.