ALOX12B Antibody

ProSci
Product Code: PSI-55-035
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-035-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in A549 cell line lysates (35ug/lane).
2 / 3
ALOX12B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis in A549 cell line lysates (35ug/lane).
ALOX12B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase, 11311-, Epidermis-type lipoxygenase 12, ALOX12B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.
Background References:
  • Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
  • Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
  • Vahlquist, A., et al. J. Invest. Dermatol. 130(2):438-443(2010)
  • Agarwal, S., et al. Exp. Dermatol. 18(11):939-946(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ALOX12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 622-652 amino acids from the C-terminal region of human ALOX12B.
NCBI Gene ID #:
242
NCBI Official Name:
Arachidonate 12-lipoxygenase, 12R-type
NCBI Official Symbol:
ALOX12B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
O75342
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
O75342
User NOte:
Optimal dilutions for each application to be determined by the researcher.