CLPX Antibody

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ProSci
Product Code: PSI-55-403
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-403-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from human heart tissue lysate, using CLPX Antibody at 1:1000 at each lane.
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CLPX antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from human heart tissue lysate, using CLPX Antibody at 1:1000 at each lane.
CLPX antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP (By similarity).
Background References:
  • Martin, A., et al. Mol. Cell 29(4):441-450(2008) Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)Kang, S.G., et al. J. Biol. Chem. 280(42):35424-35432(2005) Kang, S.G., et al. J. Biol. Chem. 277(23):21095-21102(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CLPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-477 amino acids from the C-terminal region of human CLPX.
NCBI Gene ID #:
10845
NCBI Official Name:
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
NCBI Official Symbol:
CLPX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
O76031
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
O76031
User NOte:
Optimal dilutions for each application to be determined by the researcher.