HSD17B12 Antibody

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ProSci
Product Code: PSI-63-906
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-906-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A431,SiHa cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HSD17B12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in A431,SiHa cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HSD17B12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12, 3-ketoacyl-CoA reductase, KAR, 131-, HSD17B12
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.
Background References:
  • Plourde,M. J. Steroid Biochem. Mol. Biol. 116 (3-5), 134-153 (2009)
  • Nagasaki,S. Mol. Cell. Endocrinol. 307 (1-2), 163-168 (2009)
  • Bellemare,V. J. Steroid Biochem. Mol. Biol. 114 (3-5), 129-134 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This HSD17B12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human HSD17B12.
NCBI Gene ID #:
51144
NCBI Official Name:
Estradiol 17-beta-dehydrogenase 12
NCBI Official Symbol:
HSD17B12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q53GQ0
Protein GI Number:
158931120
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q53GQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.