MTHFD1 Antibody

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ProSci
Product Code: PSI-61-504
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-504-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Confocal immunofluorescent analysis of M with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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M IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the M for immunohistochemistry.
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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Confocal immunofluorescent analysis of M with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
M IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the M for immunohistochemistry.

Documents

Further Information

Additional Names:
C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.
Background References:
  • Ivanov,A.,Am Diet Assoc 109 (2), 313-318 (2009)
  • Schmidt A., Biochemistry 39:6325-6335(2000)
  • Hol F.A., Clin. Genet. 53:119-125(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.
NCBI Gene ID #:
4522
NCBI Official Name:
C-1-tetrahydrofolate synthase, cytoplasmic
NCBI Official Symbol:
MTHFD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102 kDa
Protein Accession #:
P11586
Protein GI Number:
115206
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P11586
User NOte:
Optimal dilutions for each application to be determined by the researcher.