ADH1C Antibody

ProSci
Product Code: PSI-61-966
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-966-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western blot analysis in T47D cell line lysates (35ug/lane).
2 / 5
Western blot analysis of ADH1C using rabbit polyclonal ADH1C Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.
3 / 5
4 / 5
Confocal immunofluorescent analysis of ADH1C Antibody with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
5 / 5
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in T47D cell line lysates (35ug/lane).
Western blot analysis of ADH1C using rabbit polyclonal ADH1C Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.
Confocal immunofluorescent analysis of ADH1C Antibody with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma, ADH1C, ADH3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.
Background References:
  • Benzon Larsen, S., et al. Cancer Lett. (2010) In press :
  • Sangrajrang, S., et al. Breast Cancer Res. Treat. (2010)
  • Khan, A.J., et al. Drug Alcohol Depend (2010) In press :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ADH1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the Central region of human ADH1C.
NCBI Gene ID #:
126
NCBI Official Name:
Alcohol dehydrogenase 1C
NCBI Official Symbol:
ADH1C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
P00326
Protein GI Number:
113398
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P00326
User NOte:
Optimal dilutions for each application to be determined by the researcher.