NUP50 Antibody

ProSci
Product Code: PSI-58-676
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-676-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in WiDr cell line lysates (35ug/lane).

Western blot analysis in WiDr cell line lysates (35ug/lane).

Further Information

Additional Names:
Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L
Application Note:
For WB starting dilution is: 1:1000
Background:
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Ogawa, Y., et al. Mol. Biol. Cell 21(4):630-638(2010)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-252 amino acids from the Central region of human NUP50.
NCBI Gene ID #:
10762
NCBI Official Name:
Nuclear pore complex protein Nup50
NCBI Official Symbol:
NUP50
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q9UKX7
Protein GI Number:
20455193
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9UKX7
User NOte:
Optimal dilutions for each application to be determined by the researcher.