GATA2 Antibody

ProSci
Product Code: PSI-62-453
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-453-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GATA2 Antibody in K562 cell line lysates (35ug/lane)
2 / 3
Flow cytometric analysis of K562 cells using GATA2 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of GATA2 Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells using GATA2 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Endothelial transcription factor GATA-2, GATA-binding protein 2, GATA2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
GATA2 is a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. This protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages.
Background References:
  • Pan,X., et.al., J. Biochem. 127 (1), 105-112 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken
Immunogen:
This GATA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human GATA2.
NCBI Gene ID #:
2624
NCBI Official Name:
Endothelial transcription factor GATA-2
NCBI Official Symbol:
GATA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
P23769
Protein GI Number:
229462971
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P23769
User NOte:
Optimal dilutions for each application to be determined by the researcher.