RABGAP1 Antibody

ProSci
Product Code: PSI-55-190
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-190-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Rab GTPase-activating protein 1, GAP and centrosome-associated protein, Rab6 GTPase-activating protein GAPCenA, RABGAP1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
RABGAP1 may act as a GTPase-activating protein of RAB6A and play a role in microtubule nucleation by centrosome. It may participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Ishibashi, K., et al. Genes Cells 14(1):41-52(2009)
  • Echard, A., et al. Mol. Biol. Cell 11(11):3819-3833(2000)
  • Opdam, F.J., et al. J. Cell. Sci. 113 (PT 15), 2725-2735 (2000) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RABGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human RABGAP1.
NCBI Gene ID #:
23637
NCBI Official Name:
Rab GTPase-activating protein 1
NCBI Official Symbol:
RABGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
122 kDa
Protein Accession #:
Q9Y3P9
Protein GI Number:
156633605
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9Y3P9
User NOte:
Optimal dilutions for each application to be determined by the researcher.