YBX1 Antibody

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ProSci
Product Code: PSI-62-232
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-232-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from U-251 MG cell line, using YBX1 Antibody at 1:1000.
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Western blot analysis of YBX1 using rabbit polyclonal YBX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2).
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Fluorescent confocal image of U251 cell stained with YBX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.
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Western blot analysis of lysate from U-251 MG cell line, using YBX1 Antibody at 1:1000.
Western blot analysis of YBX1 using rabbit polyclonal YBX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2).
Fluorescent confocal image of U251 cell stained with YBX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.

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Further Information

Additional Names:
Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.
Background References:
  • Fujii,T., Cancer Res. 68 (5), 1504-1512 (2008)
  • Fraser,D.J., Kidney Int. 73 (6), 724-732 (2008)
  • Shiota,M., Cancer Res. 68 (1), 98-105 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Mouse, Rat
Immunogen:
This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1.
NCBI Gene ID #:
4904
NCBI Official Name:
Nuclease-sensitive element-binding protein 1
NCBI Official Symbol:
YBX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P67809
Protein GI Number:
54040031
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P67809
User NOte:
Optimal dilutions for each application to be determined by the researcher.