DPPA4 Antibody

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ProSci
Product Code: PSI-57-700
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-700-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining DPPA4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
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Antibody staining DPPA4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Developmental pluripotency-associated protein 4, DPPA4
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
DppA4 is a member of the Dppa family of developmental pluripotency associated transcription factors. Dppa4 is a target of Oct4, Sox2 and Nanog. It may play a role in maintaining cell pluripotentiality.
Background References:
  • Bortvin,A., Development 130 (8), 1673-1680 (2003
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DPPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human DPPA4.
NCBI Gene ID #:
55211
NCBI Official Name:
Developmental pluripotency-associated protein 4
NCBI Official Symbol:
DPPA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q7L190
Protein GI Number:
296434484
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q7L190
User NOte:
Optimal dilutions for each application to be determined by the researcher.