DDX4 Antibody

ProSci
Product Code: PSI-57-434
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-434-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western Blot at 1:1000 dilution + human ovary lysate Lysates/proteins at 20 ug per lane.
2 / 2
Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western Blot at 1:1000 dilution + human ovary lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Background References:
  • Guo,X., Asian J. Androl.(3), 339-344 (2007)
  • Zeeman,A.M., Lab. Invest. 82 (2), 159-166 (2002)
  • Castrillon,D.H., Proc. Natl. Acad. Sci. U.S.A. 97 (17), 9585-9590 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rat
Immunogen:
This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554-583 amino acids from the C-terminal region of human DDX4.
NCBI Gene ID #:
54514
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX4
NCBI Official Symbol:
DDX4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
Q9NQI0
Protein GI Number:
20138033
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9NQI0
User NOte:
Optimal dilutions for each application to be determined by the researcher.