CAPN8 Antibody

ProSci
Product Code: PSI-56-892
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-892-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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CAPN8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
CAPN8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Calpain-8, New calpain 2, nCL-2, Stomach-specific M-type calpain, CAPN8, NCL2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).
Background References:
  • Hata, S., et al. J. Biol. Chem. 282(38):27847-27856(2007)
  • Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
  • Hata, S., et al. J. Mol. Evol. 53(3):191-203(2001)
  • Huang, Y., et al. Trends Mol Med 7(8):355-362(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CAPN8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the Central region of human CAPN8.
NCBI Gene ID #:
388743
NCBI Official Name:
Calpain-8
NCBI Official Symbol:
CAPN8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
A6NHC0
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
A6NHC0
User NOte:
Optimal dilutions for each application to be determined by the researcher.