DCTN2 Antibody

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ProSci
Product Code: PSI-56-720
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-720-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from SH-SY5Y cell line, using DCTN2 Antibody at 1:1000 at each lane.
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DCTN2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from SH-SY5Y cell line, using DCTN2 Antibody at 1:1000 at each lane.
DCTN2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, DCTN-50, p50 dynamitin, DCTN2, DCTN50
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.
Background References:
  • Jacquot, G., et al. J. Biol. Chem. 285(30):23019-23031(2010)
  • Inoue, M., et al. Genes Cells 13(8):905-914(2008)
  • Maier, K.C., et al. Traffic 9(4):481-491(2008)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This DCTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids from the Central region of human DCTN2.
NCBI Gene ID #:
10540
NCBI Official Name:
Dynactin subunit 2
NCBI Official Symbol:
DCTN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q13561
Protein GI Number:
22096346
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q13561
User NOte:
Optimal dilutions for each application to be determined by the researcher.