RICTOR Antibody

ProSci
Product Code: PSI-62-771
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-771-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-RICTOR Pab in SK-BR-3 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of anti-RICTOR Pab in SK-BR-3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR {ECO:0000312|EMBL:EAW559801}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.
Background References:
  • Pearce,L.R., Biochem. J. 405 (3), 513-522 (2007)
  • Yang,Q., Genes Dev. 20 (20), 2820-2832 (2006)
  • Jacinto,E., Cell 127 (1), 125-137 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RICTOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-267 amino acids from the N-terminal region of human RICTOR.
NCBI Gene ID #:
253260
NCBI Official Name:
Rapamycin-insensitive companion of mTOR
NCBI Official Symbol:
RICTOR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
192 kDa
Protein Accession #:
Q6R327
Protein GI Number:
74710068
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q6R327
User NOte:
Optimal dilutions for each application to be determined by the researcher.