JLP Antibody

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ProSci
Product Code: PSI-62-796
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-796-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of SPAG9 using rabbit polyclonal SPAG9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of SPAG9 using rabbit polyclonal SPAG9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2).
Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, CT89, Human lung cancer oncogene 6 protein, HLC-6, JNK-associated leucine-zipper protein, JLP, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, PHET, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, SPAG9, HSS, KIAA0516, MAPK8IP4, SYD1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.
Background References:
  • Rana,R., Hum. Reprod. 21 (11), 2894-2900 (2006)
  • Rana,R., Biochem. Biophys. Res. Commun. 340 (1), 158-164 (2006)
  • Jagadish,N., KeioMed 54 (2), 66-71 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This JLP (SPAG9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-632 amino acids from the Central region of human JLP (SPAG9).
NCBI Gene ID #:
9043
NCBI Official Name:
C-Jun-amino-terminal kinase-interacting protein 4
NCBI Official Symbol:
SPAG9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
146 kDa
Protein Accession #:
O60271
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
O60271
User NOte:
Optimal dilutions for each application to be determined by the researcher.