ACTG2 Antibody

ProSci
Product Code: PSI-57-103
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-103-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).
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ACTG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate cancinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in CEM cell line lysates (35ug/lane).
ACTG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate cancinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues.
Background References:
  • Sun, Q., et al. J. Biol. Chem. 284(47):32582-32590(2009)
  • Laasanen, J., et al. Fetal. Diagn. Ther. 23(1):36-40(2008)
  • Goldberg, M.T., et al. J. Invest. Dermatol. 127(11):2645-2655(2007)
  • Yonemoto, S., et al. Clin. Exp. Nephrol. 10(3):186-192(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This ACTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-371 amino acids from the C-terminal region of human ACTG2.
NCBI Gene ID #:
72
NCBI Official Name:
Actin, gamma-enteric smooth muscle
NCBI Official Symbol:
ACTG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P63267
Protein GI Number:
54036679
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P63267
User NOte:
Optimal dilutions for each application to be determined by the researcher.