HHAT Antibody

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ProSci
Product Code: PSI-61-955
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-955-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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HHAT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562 cell line lysates (35ug/lane).
HHAT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Protein-cysteine N-palmitoyltransferase HHAT, 231-, Hedgehog acyltransferase, Melanoma antigen recognized by T-cells 2, MART-2, Skinny hedgehog protein 1, HHAT, MART2, SKI1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).
Background References:
  • Chamoun, Z., et al. Science 293(5537):2080-2084(2001)
  • Kawakami, Y., et al. J. Immunol. 166(4):2871-2877(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human HHAT.
NCBI Gene ID #:
55733
NCBI Official Name:
Protein-cysteine N-palmitoyltransferase HHAT
NCBI Official Symbol:
HHAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q5VTY9
Protein GI Number:
74747010
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q5VTY9
User NOte:
Optimal dilutions for each application to be determined by the researcher.