PUM2 Antibody

Contact us
ProSci
Product Code: PSI-63-175
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-175-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of PUM2 using PUM2 Antibody (S182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2).
2 / 3
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
❮❯

Western blot analysis of PUM2 using PUM2 Antibody (S182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Background References:
  • Kusz,K., Mol. Reprod. Dev. 74 (6), 795-799 (2007)
  • Spik,A., Reprod BiolSUPPL 1, 37-42 (2006)
  • Moore,F.L., Proc. Natl. Acad. Sci. U.S.A. 100 (2), 538-543 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from human PUM2.
NCBI Gene ID #:
23369
NCBI Official Name:
Pumilio homolog 2
NCBI Official Symbol:
PUM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
114 kDa
Protein Accession #:
Q8TB72
Protein GI Number:
41688714
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q8TB72
User NOte:
Optimal dilutions for each application to be determined by the researcher.