CPN2 Antibody

ProSci
Product Code: PSI-62-846
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-846-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CPN2 antibody in jurkat cell line lysates (35ug/lane)
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CPN2 Antibody (RB18703) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of CPN2 antibody in jurkat cell line lysates (35ug/lane)
CPN2 Antibody (RB18703) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Carboxypeptidase N subunit 2, Carboxypeptidase N 83 kDa chain, Carboxypeptidase N large subunit, Carboxypeptidase N polypeptide 2, Carboxypeptidase N regulatory subunit, CPN2, ACBP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CPN2, the 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Background References:
  • Liu,T., Qian,W.J. J. Proteome Res.(6), 2070-2080 (2005)
  • Riley,D.A., Tan,F. Genomics 50 (1), 105-108 (1998)
  • Skidgel,R.A. Biochem. Biophys. Res. Commun. 154 (3), 1323-1329 (1988)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CPN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the N-terminal region of human CPN2.
NCBI Gene ID #:
1370
NCBI Official Name:
Carboxypeptidase N subunit 2
NCBI Official Symbol:
CPN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P22792
Protein GI Number:
334302917
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P22792
User NOte:
Optimal dilutions for each application to be determined by the researcher.