MDFIC Antibody

ProSci
Product Code: PSI-63-605
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-605-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of MDFIC Antibody in Hela cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human spleen reacted with MDFIC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of MDFIC Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human spleen reacted with MDFIC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
MyoD family inhibitor domain-containing protein, I-mfa domain-containing protein, hIC, MDFIC (HGNC:28870)
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus.
Background References:
  • Thebault,S. et.al., Curr. Protein Pept. Sci.(2), 155-167 (2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MDFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-99 amino acids from the N-terminal region of human MDFIC.
NCBI Official Name:
MyoD family inhibitor domain-containing protein
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9P1T7
Protein GI Number:
257051035
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9P1T7-1
User NOte:
Optimal dilutions for each application to be determined by the researcher.