CHMP4B Antibody
| Code | Size | Price |
|---|
| PSI-63-990-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Charged multivesicular body protein 4b, Chromatin-modifying protein 4b, CHMP4b, SNF7 homolog associated with Alix 1, SNF7-2, hSnf7-2, Vacuolar protein sorting-associated protein 32-2, Vps32-2, hVps32-2, CHMP4B, C20orf178, SHAX1
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.
Background References:
- Zhou, X., et al. Biochem. J. 418(2):277-284(2009)
- McCullough, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(22):7687-7691(2008)
- Ichioka, F., et al. FEBS J. 275(4):682-692(2008)
- Hanson, P.I., et al. J. Cell Biol. 180(2):389-402(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Zebrafish, Mouse, Xenopus
Immunogen:
This CHMP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human CHMP4B.
NCBI Gene ID #:
128866
NCBI Official Name:
Charged multivesicular body protein 4b
NCBI Official Symbol:
CHMP4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
Q9H444
Protein GI Number:
24636296
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9H444
User NOte:
Optimal dilutions for each application to be determined by the researcher.