PYGO1 Antibody

ProSci
Product Code: PSI-56-267
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-267-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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PYGO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
PYGO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Pygopus homolog 1, PYGO1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
PYGO1 is involved in signal transduction through the Wnt pathway.
Background References:
  • Frank, B., et al. Carcinogenesis (2010) In press :
  • Fiedler, M., et al. Mol. Cell 30(4):507-518(2008)
  • Townsley, F.M., et al. J. Biol. Chem. 279(7):5177-5183(2004)
  • Thompson, B., et al. Nat. Cell Biol. 4(5):367-373(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PYGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-409 amino acids from the C-terminal region of human PYGO1.
NCBI Gene ID #:
26108
NCBI Official Name:
Pygopus homolog 1
NCBI Official Symbol:
PYGO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
Q9Y3Y4
Protein GI Number:
23396828
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9Y3Y4
User NOte:
Optimal dilutions for each application to be determined by the researcher.