RACGAP1 Antibody

Contact us
ProSci
Product Code: PSI-55-862
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-862-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
2 / 4
RACGAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 4
4 / 4
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Western blot analysis in MCF-7 cell line lysates (35ug/lane).
RACGAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Rac GTPase-activating protein 1, Male germ cell RacGap, MgcRacGAP, Protein CYK4 homolog, CYK4, HsCYK-4, RACGAP1 (HGNC:9804)
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009)
  • Simon, G.C., et al. EMBO J. 27(13):1791-1803(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RACGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human RACGAP1.
NCBI Gene ID #:
29127
NCBI Official Name:
Rac GTPase-activating protein 1
NCBI Official Symbol:
RACGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
Q9H0H5
Protein GI Number:
74762727
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q9H0H5
User NOte:
Optimal dilutions for each application to be determined by the researcher.