HLA-DRB4 Antibody
| Code | Size | Price |
|---|
| PSI-57-203-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
HLA class II histocompatibility antigen, DR beta 4 chain, MHC class II antigen DRB4, HLA-DRB4
Application Note:
For WB starting dilution is: 1:1000
Background:
HLA-DRB4 belongs to the HLA class II beta chain
paralogues. This class II molecule is a heterodimer consisting of
an alpha (DRA) and a beta (DRB) chain, both anchored in the
membrane. It plays a central role in the immune system by
presenting peptides derived from extracellular proteins. Class II
molecules are expressed in antigen presenting cells (APC: B
lymphocytes, dendritic cells, macrophages). The beta chain is
approximately 26-28 kDa and its gene contains 6 exons. Exon one
encodes the leader peptide, exons 2 and 3 encode the two
extracellular domains, exon 4 encodes the transmembrane domain and
exon 5 encodes the cytoplasmic tail. Within the DR molecule the
beta chain contains all the polymorphisms specifying the peptide
binding specificities. Typing for these polymorphisms is routinely
done for bone marrow and kidney transplantation. DRB1 is expressed
at a level five times higher than its paralogues DRB3, DRB4 and
DRB5. The presence of DRB4 is linked with allelic variants of DRB1,
otherwise it is omitted. There are 4 related pseudogenes: DRB2,
DRB6, DRB7, DRB8 and DRB9.
Background References:
- Awa, W.L., et al. Eur. J. Endocrinol. 163(1):97-104(2010)
- Christiansen, O.B., et al. J. Reprod. Immunol. 85(1):9-14(2010)
- Amoli, M.M., et al. Dis. Markers 28(1):49-53(2010)
- Gomez-Tortosa, E., et al. Dement Geriatr Cogn Disord 30(1):8-11(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HLA-DRB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human HLA-DRB4.
NCBI Gene ID #:
3126
NCBI Official Name:
HLA class II histocompatibility antigen, DR beta 4 chain
NCBI Official Symbol:
HLA-DRB4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P13762
Protein GI Number:
281371554
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P13762
User NOte:
Optimal dilutions for each application to be determined by the researcher.