RFX5 Antibody
| Code | Size | Price |
|---|
| PSI-57-412-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA-binding protein RFX5, Regulatory factor X 5, RFX5
Application Note:
For WB starting dilution is: 1:1000
Background:
A lack of MHC-II expression results in a severe
immunodeficiency syndrome called MHC-II deficiency, or the bare
lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation
groups have been identified in B-cell lines established from
patients with BLS. The molecular defects in complementation groups
B, C, and D all lead to a deficiency in RFX, a nuclear protein
complex that binds to the X box of MHC-II promoters. The lack of
RFX binding activity in complementation group C results from
mutations in the RFX5 gene encoding the 75-kD subunit of RFX
(Steimle et al., 1995). RFX5 is the fifth member of the growing
family of DNA-binding proteins sharing a novel and highly
characteristic DNA-binding domain called the RFX motif. Multiple
alternatively spliced transcript variants have been found but the
full-length natures of only two have been determined. [provided by
RefSeq].
Background References:
- Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010)
- Kong, X., et al. J. Mol. Cell. Cardiol. 46(3):292-299(2009)
- Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)
- Ennis, S., et al. Lancet 372(9652):1828-1834(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RFX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human RFX5.
NCBI Gene ID #:
5993
NCBI Official Name:
DNA-binding protein RFX5
NCBI Official Symbol:
RFX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
P48382
Protein GI Number:
1350587
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P48382
User NOte:
Optimal dilutions for each application to be determined by the researcher.