ProSci

NFIL3 Antibody

Product Code:
 
PSI-58-654
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).

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PSI-58-654-400ul400ul£626.00
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Further Information

Additional Names:
Nuclear factor interleukin-3-regulated protein, E4 promoter-binding protein 4, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1, Transcriptional activator NF-IL3A, NFIL3, E4BP4, IL3BP1
Application Note:
For WB starting dilution is: 1:1000
Background:
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Weng, Y.J., et al. Mol. Cell. Biochem. 340 (1-2), 187-194 (2010) :
  • Lavebratt, C., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (2), 570-581 (2010) :
  • Utge, S.J., et al. PLoS ONE(2), E9259 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat, Xenopus
Immunogen:
This NFIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human NFIL3.
NCBI Gene ID #:
4783
NCBI Official Name:
Nuclear factor interleukin-3-regulated protein
NCBI Official Symbol:
NFIL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q16649
Protein GI Number:
150385077
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q16649
User NOte:
Optimal dilutions for each application to be determined by the researcher.