HLA-DQA1 Antibody
| Code | Size | Price |
|---|
| PSI-56-292-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, HLA-DQA1
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
HLA-DQA1 belongs to the HLA class II alpha chain
paralogues. The class II molecule is a heterodimer consisting of an
alpha (DQA) and a beta chain (DQB), both anchored in the membrane.
It plays a central role in the immune system by presenting peptides
derived from extracellular proteins. Class II molecules are
expressed in antigen presenting cells (APC: B Lymphocytes,
dendritic cells, macrophages). The alpha chain is approximately
33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader
peptide, exons 2 and 3 encode the two extracellular domains, and
exon 4 encodes the transmembrane domain and the cytoplasmic tail.
Within the DQ molecule both the alpha chain and the beta chain
contain the polymorphisms specifying the peptide binding
specificities, resulting in up to four different molecules. Typing
for these polymorphisms is routinely done for bone marrow
transplantation.
Background References:
- Donaldson, P.T., et al. J. Hepatol. 53(6):1049-1053(2010)
- Noble, J.A., et al. Diabetes 59(11):2972-2979(2010)
- Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010)
- Ovsyannikova, I.G., et al. PLoS ONE(7), E11806 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HLA-DQA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the Central region of human HLA-DQA1.
NCBI Gene ID #:
100509457, 3117
NCBI Official Name:
HLA class II histocompatibility antigen, DQ alpha 1 chain
NCBI Official Symbol:
HLA-DQA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
P01909
Protein GI Number:
122188
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P01909
User NOte:
Optimal dilutions for each application to be determined by the researcher.