ZBTB7B Antibody

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ProSci
Product Code: PSI-62-264
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-264-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Zinc finger and BTB domain-containing protein 7B, Krueppel-related zinc finger protein cKrox, hcKrox, T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 15, Zinc finger protein 67 homolog, Zfp-67, Zinc finger protein 857B, Zinc finger protein Th-POK, ZBTB7B, ZBTB15, ZFP67, ZNF857B
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.
Background References:
  • Galera,P., Proc. Natl. Acad. Sci. U.S.A. 91 (20), 9372-9376 (1994)
  • Widom,R.L., Gene 198 (1-2), 407-420 (1997)
  • Heegaard,A.M., J. Bone Miner. Res. 12 (12), 2050-2060 (1997)
  • Widom,R.L., Matrix Biol. 20 (7), 451-462 (2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-469 amino acids from the C-terminal region of human ZBTB7B.
NCBI Gene ID #:
51043
NCBI Official Name:
Zinc finger and BTB domain-containing protein 7B
NCBI Official Symbol:
ZBTB7B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
O15156
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
O15156
User NOte:
Optimal dilutions for each application to be determined by the researcher.